100 micron floating sections

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From:Nperson211@aol.com (by way of histonet)
To:histonet <histonet@magicnet.net>
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Does anyone have any sure fire tips for immunostaining 100 micron floating
sections so that the antibodies actually penetrate?  Triton,
saponin...whatever?  Any comments or suggestions will be appreciated.
Thanks Nancy Lemke

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