layered structures

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From:Katrina Knott <> (by way of histonet)
To:histonet <>
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	I have been having problems with sectioning layered structures such as
stomach and skin.  I read that as paraffin cools it can push the tissue
together creating folds.  As I section I get fold within the tissue (usually
within the epithelium layer) that can not be manipulated on the water bath.
Does anyone have any suggestions on how to get around this problem, ie.
change of knife angle, hotter water bath, etc.??


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