frozen fixation

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From:Gayle Callis <> (by way of histonet)
To:histonet <>
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After using 95% ethanol for years, Iswitched to coplin jar
of NBF (alcoholic formalin would probably work just as well).
fixation is approx 15 - 30 sec for a 5 um frozen, rinsed with tap water
and stained, never lost a renal biopsy section.  Frozens for lipids
are fantastic.

The morphology was better, more like the paraffin sections, staining
definitely looked better with a rapid H&E.  Only drawback is keeping
fumes to zero, but never noticed them if screw cap/covered jar is used.
all rinsing, staining etc iis done n afume hood.

My problem with sprays is the aerosol, and what I am breathing, plus you
are spraying onto potential biohazardous material, does this cause
aerosol that is dangerous to you?   Always a niggling thought, that is
why our tissue freezing is never done with a cryospray.  Peggy Wenk
might comment on this---------

Hope this helps
Gayle Callis

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