frozen fixation
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From: | Gayle Callis <uvsgc@msu.oscs.montana.edu> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
After using 95% ethanol for years, Iswitched to coplin jar
of NBF (alcoholic formalin would probably work just as well).
fixation is approx 15 - 30 sec for a 5 um frozen, rinsed with tap water
and stained, never lost a renal biopsy section. Frozens for lipids
are fantastic.
The morphology was better, more like the paraffin sections, staining
definitely looked better with a rapid H&E. Only drawback is keeping
fumes to zero, but never noticed them if screw cap/covered jar is used.
all rinsing, staining etc iis done n afume hood.
My problem with sprays is the aerosol, and what I am breathing, plus you
are spraying onto potential biohazardous material, does this cause
aerosol that is dangerous to you? Always a niggling thought, that is
why our tissue freezing is never done with a cryospray. Peggy Wenk
might comment on this---------
Hope this helps
Gayle Callis
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