freezing tissue for frozens

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From:"Instrumedics, Inc." <> (by way of histonet)
To:histonet <>
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We have found that to retain the morphology of all tissues it is important
to minimize the growth of ice crystals. Slow freezing results in ice crystal
growth. These large ice crystals can be very damaging to the cells, poking
holes in membranes. Snap freezing minimizes ice crystal growth.
The Gentle Jane snap freezing method  produces "amorphous" ice. This depends
on the temperature and thermal exchange  With the Gentle Jane a 12oz.,
mirror-finished,chrome plated heat extractor is  chilled in liquid nitrogen
to -196degrees Celsius. It is then lowered on the Gentle Jane and contacts
the tissue and embedding medium. In 8-10 seconds the block is ready for
sectioning. The tissue is uppermost in the block minimizing the trimming
step. If then the CryoJane process is used the frozen section will be
paraffin-quality or better.


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