Re Diastase/Amylase

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From:bbracing <> (by way of histonet)
To:histonet <>
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Many thanks from myself and my staff to all of you who answered my query on
Diatase/Amylase digestion of Glycogen.  We tried many of the sugestions,
using newly purchased products, with considerable success.  After several
small blind studies on different control sections, some digested with
saliva, others with Amylase formulated as sugested, we settled on the
following.  A  0.2% aquous solution of amylase (in distilled water, sigma
A3176) for 20 min at room temp.  This gave us the convenience of use that
fit our work pattern, and in the blind study no one was able to detect
which slides were digested by what.
So now to everyones relief -- No More Spitting!.
Kerry Beebe
Kelowna Gen Hospital
Kelowna B.C. Canada

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