Katrina- Try a hotter water bath. Jeff Silverman

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> From: Katrina Knott <knottk@amc.org>
> To: histonet <histonet@pathology.swmed.edu>
> Subject: sectioning
> Date: Saturday, February 06, 1999 1:17 PM
>
> I have been having problems with sectioning layered structures such as
> stomach and skin.  I read that as paraffin cools it can push the tissue
> together creating folds.  As I section I get fold within the tissue
(usually
> within the epithelium layer) that can not be manipulated on the water
bath.
> Does anyone have any suggestions on how to get around this problem, ie.
> change of knife angle, hotter water bath, etc.??
>
> Thanks,
> Katrina
>