Re: sectioning

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From:Mick Rentsch <> (by way of histonet)
To:histonet <>
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Dear Katrina,
the advice from Kerry of Canada is spot on. I would also add that you may
also have a severe compression problem. Adjust your knife angle so that the
bevel just clears the face of the block. Pre-chill your blocks by placing
them on ice water or a block of ice for ten or more minutes, run off your
sections and put it back on ice to chill for more. Properly processed and
infiltrated tissue shouldn't give you any problems, if things persist, have
a good look at your processor maintainance schedule.
Regards Mike (Downunder)
-----Original Message-----
From: Katrina Knott <>
To: histonet <>
Date: Sunday, 7 February 1999 5:37
Subject: sectioning

>I have been having problems with sectioning layered structures such as
>stomach and skin.  I read that as paraffin cools it can push the tissue
>together creating folds.  As I section I get fold within the tissue
>within the epithelium layer) that can not be manipulated on the water bath.
>Does anyone have any suggestions on how to get around this problem, ie.
>change of knife angle, hotter water bath, etc.??

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