Re: histo. of the Cochlear

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From:Katie B <bresee98@yahoo.com> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Karen--

How big are these tissues when you decalcify them in EDTA?  I'm
curious if the tissues I work with can go through your recipe for EDTA
and be done with faster than what I'm doing now.  Presently, I have to
wait 4 days to a week and do it at 4 degrees.

-Katie Bennett



---Karen S Pawlowski <kna101@utdallas.edu> wrote:

> As for decalcification, you can do TEM, SEM, and LM without it, but
that
> gets pretty tricky.  When I decalcifiy, I use an EDTA mixture for a
day or
> two.  The recipe I use for this now works faster, with no difference
in
> arcitecture from the recipe I used to use.  Here is the recipe:
>
> 291.1 gm EDTA (Sigma #ED4SS)
> 1 L distilled water
> mix and add about 42 ml of acetic acid to bring pH to 8.0
> Bring total volume to 2 L
>
> I decalcify at room temp.
>


==
Catherine "Katie" Bresee Bennett
Laboratory for Experimental Pathology
Department of Veterinary Pathology
Michigan State University

*new* e-mail: bresee98@yahoo.com

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