Re: histo. of the Cochlear

<< Previous Message | Next Message >>
From:Katie B <> (by way of histonet)
To:histonet <>
Content-Type:text/plain; charset="us-ascii"


How big are these tissues when you decalcify them in EDTA?  I'm
curious if the tissues I work with can go through your recipe for EDTA
and be done with faster than what I'm doing now.  Presently, I have to
wait 4 days to a week and do it at 4 degrees.

-Katie Bennett

---Karen S Pawlowski <> wrote:

> As for decalcification, you can do TEM, SEM, and LM without it, but
> gets pretty tricky.  When I decalcifiy, I use an EDTA mixture for a
day or
> two.  The recipe I use for this now works faster, with no difference
> arcitecture from the recipe I used to use.  Here is the recipe:
> 291.1 gm EDTA (Sigma #ED4SS)
> 1 L distilled water
> mix and add about 42 ml of acetic acid to bring pH to 8.0
> Bring total volume to 2 L
> I decalcify at room temp.

Catherine "Katie" Bresee Bennett
Laboratory for Experimental Pathology
Department of Veterinary Pathology
Michigan State University

*new* e-mail:

Get your free address at

<< Previous Message | Next Message >>