Katie,

The cochleae that I decalcify overnite or 24 hrs. are from mice, guinea
pigs, and chinchilla.  The tissue is rather small, about 8 mm X 3 mm X 3
mm, at maximum and the tissue is placed on a rotator to decalcify.  The
cochlear duct lies within this bony capsule, so it is not solid bone.
This not the fastest decal. out there I'm sure, but it preserves the
histology well enough for TEM.

Karen

On Tue, 9 Feb 1999, Katie B wrote:

> Karen--
>
> How big are these tissues when you decalcify them in EDTA?  I'm
> curious if the tissues I work with can go through your recipe for EDTA
> and be done with faster than what I'm doing now.  Presently, I have to
> wait 4 days to a week and do it at 4 degrees.
>
> -Katie Bennett
>
>
>
> ---Karen S Pawlowski <kna101@utdallas.edu> wrote:
>
> > As for decalcification, you can do TEM, SEM, and LM without it, but
> that
> > gets pretty tricky.  When I decalcifiy, I use an EDTA mixture for a
> day or
> > two.  The recipe I use for this now works faster, with no difference
> in
> > arcitecture from the recipe I used to use.  Here is the recipe:
> >
> > 291.1 gm EDTA (Sigma #ED4SS)
> > 1 L distilled water
> > mix and add about 42 ml of acetic acid to bring pH to 8.0
> > Bring total volume to 2 L
> >
> > I decalcify at room temp.
> >
>
>
> ==
> Catherine "Katie" Bresee Bennett
> Laboratory for Experimental Pathology
> Department of Veterinary Pathology
> Michigan State University
>
> *new* e-mail: bresee98@yahoo.com
>
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