Re: bcl2
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From: | John Bliss <bliss@zymed.com> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Dear H. Grabsch:
Zymed Laboratories offers the Bcl-2-100 mouse monoclonal antibody that has
been used with good results on paraffin-embedded (PE) tissues (Pezzella et
al; Am J Pathol 137(2):225-232 (1990). We recommend epitope retrieval in a
boiling bath, citrate buffer, pH 6.0, for 10 min. as a pretreatment.
Bcl-2-100 is available as a concentrate, predilute, and V-Line predilute.
Please contact us or your local Zymed distributor for more details.
Sincerely,
John Bliss
Zymed Laboratories, Inc
458 Carlton Court
So. San Francisco, CA 94080
Tel: 800-871-4494
FAX: 650-871-4499
E-mail: histo@zymed.com
Website: www.zymed.com
--
Heike Grabsch wrote:
> Urgent help needed!
> for months we did immmunohistochemistry on formalin-fixed,
> paraffin-embedded tissues for bcl2 with no problems. We use the
> monoclonal from Dako, dilution 1:40, 5 min pressure cooking in citrate
> buffer pH 6.0
> And now, all over sudden, it does not work anymore, only very very faint
> staining. We checked dilutions and even tried antibody "pure". Expiration
> date of the lot is 2001.
> Any suggestions? How do you pretreat your tissue sections for bcl2?
>
> thanks for your help,
>
> Heike Grabsch, Germany
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