-----Original Message-----
From: gp <elephantsneedus@erols.com>
To: histonet@pathology.swmed.edu <histonet@pathology.swmed.edu>
Date: February 14, 1999 8:34 AM
Subject: Sirius Red method for amyloid


>Does anyone have a shortened protocol for Sirius Red for amyloid.
>
>Thanks!
>
>Marian
>
>

Sirius red can be used in a Highman's congo red type procedure quite
effectively, as can many other of the "sirius" direct cotton dyes.  Total
time to stain is about 15 minutes.

Solutions
0.5% Congo red (or Sirius red) in 50% ethanol
0.2% potassium hydroxide in 70% ethanol
Mayers alum hematoxylin or another progressive type.


Method
1.    Sections to water.
2.    Stain nuclei for 2 minutes, no need to blue.
3.    Place in dye solution for 5 minutes or longer.
4.    Rinse with water.
5.    Place into alkaline ethanol until dye ceases to extract - a few
minutes.
6.    Rinse well with water.
7.    Dehydrate, clear and mount.

Amyloid - red
Nuclei - blue


Bryan Llewellyn