Re: Reagent or Blended Alcohols

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From:Jeff Silverman <> (by way of histonet)
To:histonet <>
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I've used Histoclear, HemoDe, Clearium, Americlear interchangeably for nine
years for staining H&E, specials, immunos, and PAPs with no bad effects. I
don't use it however in the tissue processor, here I still use xylene
because it appears to be a far superior clearing agent for processing. No
problems with sensitization or slide fading, provided slides aren't left in
bright sunlight for prolonged periods. You can coverslip direct from
Histoclear et al but need xylene to clean up smudged slides or remove
coverslips. Everyone here seems to like the orange smell and I even found a
reference that reports that D-limonene has anticancer activity!. I know
that some people hate the smell and are sensitized, guess I'm just lucky.
Jeff Silverman
Southampton Hospital NY USA

> From: Patrick M. Haley  <>
> To: Histonet <>
> Subject: Reagent or Blended Alcohols
> Date: Friday, February 05, 1999 3:17 PM
> I am curious about the pros and cons of using reagent and/or blended
> alcohol's for tissue processing and staining. Does anyone out there in
> HistoCyberLand use these reagents?
> I welcome all comments and advice.
> Also, any users of Histoclear or FisherBrand Hemo-De Clearing agents? How
> do these perform for you?
> Thank You!!
> Pat

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