Re: Fatty Specimens

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From:"R.Wadley" <s9803537@pop3.unsw.edu.au> (by way of histonet)
To:histonet <histonet@magicnet.net>
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	Dear Jennifer,

	I am not surprised the soapy water suggestion is working.  Several
years
ago it was recommended to me as a medium in which to mount pathology museum
display pieces.  Unfortunately, I no longer work in that field so have been
unable to verify it for myself.  As to being able to do immuno's, I cannot
comment as I am unsure of the fixation process involved.  I'm afraid it
have to be a case of "suck it & see".
	As to the constant desire of pathologists to get things done
yesterday, I
can only suggest that in the last modern hospital lab I worked in,
microwave fixation proved an ideal solution.

	Regards

	Rob W.

At 03:15 PM 2/9/99 -0700, you wrote:
>The discussion last week about the different methods to remove excess
>fat had me anxious to try the soapy water suggestion. It seemed to
>simple to be true. Well it has worked great but now a new question has
>come up. Has anyone used this method and then done immuno's on the
>tissue? Was their any difference? If this wont be a problem I have to
>say I love the results so far. Out Doc's seem to think that no matter
>when the specimen gets to the lab and no matter what kind on specimen it
>is it HAS to make it on the processor that night. Needless to say the
>sections often look a bit unfixed.
>
>Jennifer Saunders H.T.
>
>
>
>
R. Wadley, B.App.Sc, M.L.S
Laboratory Manager
Cellular Analysis Facility
School of Microbiology & Immunology
UNSW 2052
Ph (BH) 	+61 (2) 9385 3517
Ph (AH)	+61 (2) 9555 1239
Fax 	+61 (2) 9385 1591
E-mail	r.wadley@unsw.edu.au
www	http://www.unsw.edu.au/clients/microbiology/CAF.html
	(Under development)




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