Re: Fast or Slow Freezing

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From:"R.Wadley" <> (by way of histonet)
To:histonet <>
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	Generally speaking it is always better to freeze rapidly.  The
at which you section the tissue in the cryostat will depend on type of
tissue, size, moisture content, how thick or thin you want the sections,
etc.  I can send you some references, it might take me a couple of days to
find them.  Slow freezing, particularly with high moisture content tissues
results in significant ice crystal formation & accompanying morphological
damage at both cellular & intra cellular levels.

At 02:47 PM 2/8/99 -0500, you wrote:
>In doing frozen sections, is it better to freeze fast or slow? Is this
>determined by the type of tissue, size, moisture content or some other
>criteria? Please reply as you see fit, either direct to me or to the net.

R. Wadley, B.App.Sc, M.L.S
Laboratory Manager
Cellular Analysis Facility
School of Microbiology & Immunology
UNSW 2052
Ph (BH) 	+61 (2) 9385 3517
Ph (AH)	+61 (2) 9555 1239
Fax 	+61 (2) 9385 1591
	(Under development)

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