Re: Demonstration of amylase

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From:Mick Rentsch <> (by way of histonet)
To:histonet <>
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Dear Jim,
I know I sound niave, but what would happen if you applied schiff reagent to
the section (No Oxidation Step), gave it a good rinse in 0.5% Sodium
Metabisulphite (Fresh) and then examined it. Wouldn't work would it, because
no sugar, mucin or glycogen necessarily at site, and if was incorporated in
solution before application of schiff would have to bind to site  and be
Could you use an anti-amylase generated antibody  if there is such a thing?
with a 1-5 glycosidic linkage sugar attached, then the glycogen like
material would be bound to the site and visualise with the schiff reagent.
My suggestion is probably pie in the sky stuff, but who knows?
Regards Mike (Downunder)
-----Original Message-----
From: Jim Almond <>
To: <>
Date: Thursday, 11 February 1999 4:50
Subject: Demonstration of amylase

>Hi everyone
>We have been asked to demonstrate sites of amylase activity on frozen
>tissue sections. Help would be appreciated if anyone has a reliable method.
>An initial trial of starch film methods is causing problems with
>reproducibility. Any tips, advice or alternative approaches gratefully
>Jim Almond
>Royal Shrewsbury Hospital
>Jim Almond
>Tel: +44 (0) 1743 261168  Fax: +44 (0) 1743 355963

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