Re: Choice of fixative

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We have been doing BrdU IHC for cell proliferation here for years.  We use
10% NBF fixing the tissues 24-48 hours.  I would also suggest that for
studing intestinal cell proliferation that you use an one hour IP
injection of BrdU opposed to the implantable minipump.

-- Begin original message --

> Dear histonetters,
> We would like to assess intestinal cell proliferation and migration
> in animals treated with BrDu-immunocytochemistry will be used. Does it
> matter whether the tissue is fixed in Bouin's, Carnoy's or Formalin?
> Thanks in advance for your comments.
> Deshanie Ganessunker
> University of Illinois-Urbana-Champaign
-- End original message --

best regards,
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
Chapel Hill, NC 27599
office 919-966-6343
   Lab 919-966-6140
   Fax 919-966-6123

**Suppose you were an idiot... And suppose you were a member of Congress
But I repeat myself.-Mark Twain**

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