Mike, We cut lengthwise sections of rat tibias using an IsoMet Low Speed
Diamond Saw from Buehler, however, we're not doing this for cultures so I
don't know if you could maintain sterile conditions. If you don't mind
occasional cracking of the bone I find you can do a pretty good job just
using single edged razor blades so you could use a new sterile blade for
each bone.
John

> ----------
> From: 	Mike Bromley[SMTP:MBromley@PICR.man.ac.uk]
> Sent: 	Thursday, February 04, 1999 12:33 AM
> To: 	'histonet@pathology.swmed.edu'
> Subject: 	slicing rat tibia
>
> Dear All,
> I have been asked by one of our research workers to think of a way of
> slicing rat tibia lengthways to expose the growth plate so that the
> bones can then be put into culture.  Does anyone have any ideas or
> know of any devices that would enable him to do this?
>
> Mike
>
> Michael Bromley PhD
> Histology Department
> Paterson Institute
> Wilmslow Road
> Manchester M20 9BX
> UK
>
>
>