Interleukin (IL)-8 is a multifunctional cytokine that stimulates the
division of endothelial cells. ISH and immunohistochemical analysis reveals
that IL-8 is localized in the cytoplasm of gastric tumor cells. Try a
formalin-fixed, paraffin embedded gastric carcinoma. I have also seen
lymphocytic staining in hyperplastic palatine tonsil.


Eric Kellar
Histology/Immunohistochemistry
University of Pittsburgh Medical Center




	----------
	From:  Mark Elliott [SMTP:MElliott@prl.pulmonary.ubc.ca]
	Sent:  Tuesday, February 09, 1999 10:19 AM
	To:  HistoNet@Pathology.swmed.edu
	Subject:  IL-8 control tissue

	Was wondering if anyone could recommend a good positive control
tissue for use with
	an IL-8 antibody.  We are trying to get the antibody to stain
formalin-fixed, paraffin
	embedded tissue but are not sure what tissue to use as control to
give good positive
	staining.  Any help greatly appreciated.

	Thanks


	Mark Elliott
	UBC-Pulmonary Research Lab
	St. Paul's Hospital
	Vancouver, BC Canada V6Z 1Y6
	604-631-5351 (fAX)