Andrea,
	I think there are probably as many techniques as people in
histoland. There are some nice articles by Shi and others correlating temp,
time and pH all important factors. If you have access to Pub Med just enter
Shi, if you would like exact references I can look them up for you.
Cynthia Favara
NIAID/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
PH: 406-363-9317
FAX: 406-363-9286


-----Original Message-----
From: andreah@imclone.com [mailto:andreah@imclone.com]
Sent: Tuesday, February 09, 1999 1:14 PM
To: HistoNet@Pathology.swmed.edu
Subject: Heat retrieval - the basics?


When using a microwave for heat antigen retrieval in citrate buffer how
does one prevent the evaporation and bubbling?  What containers do
people out there use for such a procedure?  Any special hints?

Thanks in advance for your input.
Andrea
AndreaH@imclone.com