RE: FISH in paraffin
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From: | "Connolly, Brett" <brett_connolly@merck.com> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Heike,
You can look at the HER-2/neu studies, Cancer Res 54:5675-5682,1994 ; Cancer
Res 55:5693-5698, 1995; J Clin Oncol 15:2894-2904, 1997 among others.
Brett
Brett M. Connolly, Ph.D.
Merck Research Laboratories
Dept. of Human Genetics
WP26A-3000
P.O. Box 4
Sumneytown Pike & Broad St.
West Point, PA 19486
ph.215-652-2501
FAX. 215-652-2075
email:brett_connolly@merck.com
> ----------
> From: Heike Grabsch
> Sent: Thursday, February 11, 1999 1:36 AM
> To: histonet@pathology.swmed.edu
> Subject: FISH in paraffin
>
> We would like to establish FISH technique for cytogenetic studies in
> formalin fixed human paraffin materials. F.ex. I want to use a panel of
> cnetromeric probes and see in paraffin sections how many chromosomes are
> in the nucleus of this particular cell and the neighbouring cells.
>
> Anybody out there in the histoland who has done FISH on paraffin
> material? and can direct me to protocols which work? Most of the
> literature is about doing FISH in cells but not tissue sections.
>
> thanks for your help,
>
> Heike Grabsch, Germany
>
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