Tammy,

Let you pathologist know that BioFix is manufactured by Accra and available
in 50 ml spray pump from Columbia Diagnostics, Inc.1-800-828-4375.
It contains ethanol, acetone and carbowax.

Eric Kellar
Histology/Immunohistochemistry
University of Pittsburgh Medical Center


	----------
	From:  Tammy Bird [SMTP:VVTBIRD@ihc.com]
	Sent:  Thursday, February 11, 1999 9:09 AM
	To:  histonet@pathology.swmed.edu
	Subject:  Cytospray fixation of frozen sections

	The following message was received on a Pathology list server that
our
	Pathologist monitors.  It has not received any responses as of yet
and
	my Pathologist ask me to post it here.  He is interested in trying
it.

	Crescenzi et. al. reported in the January '99 issue of Modern
Pathology
	that the use of a cytology spray fixative called BIO-FIX on frozen
	sections provided superior morphologic detail than immersion in 95%
	ethanol prior to staining.

	Can anyone tell me who distributes BIO-FIX in the U.S.?

	Can anyone familiar with this product tell me if it's contents are
	similar to other commonly used spray fixatives for cytology smears?

	The authors do not specify how long the spray fixative is left on
the
	frozen section, nor do they indicate specifically how the spray
fixative
	is removed.  They simply indicate that they were hydrated and
stained
	with H&E and claim that morphologically, their sections were
comparable
	to formalin fixed paraffin sections.

	Thanks in advance for your responses.

	Tammy