FW: frozen fixation
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From: | mark.lewis@shandon.com (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
FYI
Shandon has a fixative made just for frozen sections. It's called Rapid fixx.
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From: uvsgc@msu.oscs.montana.edu [SMTP:PC :uvsgc@msu.oscs.montana.edu]
Sent: Tuesday, February 09, 1999 3:48 PM
To: histonet@pathology.swmed.edu
Subject: frozen fixation
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After using 95% ethanol for years, Iswitched to coplin jar of NBF
(alcoholic formalin would probably work just as well). fixation is approx
15 - 30 sec for a 5 um frozen, rinsed with tap water and stained, never
lost a renal biopsy section. Frozens for lipids are fantastic.
The morphology was better, more like the paraffin sections, staining
definitely looked better with a rapid H&E. Only drawback is keeping fumes
to zero, but never noticed them if screw cap/covered jar is used. all
rinsing, staining etc iis done n afume hood.
My problem with sprays is the aerosol, and what I am breathing, plus you
are spraying onto potential biohazardous material, does this cause aerosol
that is dangerous to you? Always a niggling thought, that is why our
tissue freezing is never done with a cryospray. Peggy Wenk might comment
on this---------
Hope this helps
Gayle Callis
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