FISH in paraffin

<< Previous Message | Next Message >>
From:Heike Grabsch <> (by way of histonet)
To:histonet <>
Content-Type:text/plain; charset="us-ascii"

We would like to establish FISH technique for cytogenetic studies in
formalin fixed human paraffin materials. F.ex. I want to use a panel of
cnetromeric probes and see in paraffin sections how many chromosomes are
in the nucleus of this particular cell and the neighbouring cells.

Anybody out there in the histoland who has done FISH on paraffin
material? and can direct me to protocols which work? Most of the
literature is about doing FISH in cells but not tissue sections.

thanks for your help,

Heike Grabsch, Germany

<< Previous Message | Next Message >>