DAB precipitate

<< Previous Message | Next Message >>
From:tjohnso@nimr.mrc.ac.uk (Tammaryn Johnson) (by way of histonet)
To:histonet <histonet@magicnet.net>
Reply-To:
Content-Type:text/plain; charset="us-ascii"

Hi Everyone,

Please can anyon ehelp to solve a mystery. I had finished some
immunostaining and was dehydrating my slides when all my DAB precipitate
dissappeared??

After repeating the staining and abit of detective work I found out it was
the 70% alcohol I was using to dehydrate that was taking out my DAB.

Can anybody explain what might have contaminated the 70% alcohol to cause
this problem.

Thanks
Tammaryn

National Institute of Medical Research,
Department of Neurobiology,
The Ridgeway,
Mill Hill,
London,
England.
FAX 0181 913 8523
TEl 0181 959 3666 ext 2391




<< Previous Message | Next Message >>