Crescenzi et. al. reported in the January '99 issue of Modern
Pathology that the use of a cytology spray fixative called BIO-FIX
on frozen sections  provided superior morphologic detail than
immersion in 95% ethanol prior to staining.

Can anyone tell me who distributes BIO-FIX in the U.S. ?

Can anyone familiar with this product tell me if it's contents
are similar to other commonly used spray fixatives for cytology
smears?

The authors do not specify how long the spray fixative is left on the
frozen section, nor do they indicate specifically how the spray
fixative is removed. They simply indicate that they were hydrated and
stained with H&E and claim that morphologically, their sections
were comparable to formalin fixed paraffin sections.

Comments on this method of fixing frozen sections prior to staining ?
******************************************************************
Vinnie Della Speranza
Technical Director
Anatomic Pathology Laboratories
University Hospital & Medical Center
State University of New York at Stony Brook 11794-7025
(516) 444-8249
fax: (516) 444-3419
vdella@path.som.sunysb.edu