Re: [Histonet] frozen slides storage

From:"Wynn, Carmen"

Hello Ann,

I have experienced that problem as well.  I work in a research lab that
focuses on mouse and rat tissue so I obtained colored micro slide boxes
that I label on both the face and hinge side.  I store my boxes with the
hinge side facing the door so that I can maximize space.  In my case I
used green color frost plus microscope slides and green boxes for mouse
tissue and red color frost microscope plus slides and red boxes for rat
tissue.  It works out nice for me because when the frost builds up in
the freezer at least you can narrow down the tissue type and area just
by looking for colors. I keep mouse tissue on one shelf and rat on
another.  The tissue that I am currently working with is always kept in
the front so that I can reach it quickly without setting off the freezer
alarm and letting to much heat in.  It worked so well for me that I
store all my paraffin samples the same way.  I have seen at least 2
other colors of boxes available....Blue and Yellow.  The boxes and
slides are available thru Fisher or VWR.  However, the cheapest micro
slide box vendor that I have used is Lab Storage Systems (I paid
$6.45/box).  I also have gotten great deals on Color Frost and Color
frost Plus slides from Lab Source (in IL).  I hope this helps otherwise
you should in vest in a good set of really thick mittens for those cold

Carmen Wynn, M.S., Senior Scientist
Astellas Research Institute of America, LLC. (ARIA)
Illinois Science and Technology Park
8045 Lamon Ave
Skokie, IL 60077
Direct: 847-933-7419
Main: 847-933-7400
Fax: 847-933-7401 

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Sent: Friday, February 29, 2008 12:09 PM
Subject: Histonet Digest, Vol 51, Issue 44

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Today's Topics:

   1. Re: Bone Marrow Issues (Rene J Buesa)
   2. Re: frozen slides storage (Rene J Buesa)
   3. Re: frozen slides storage (Rene J Buesa)
   4. Susie Hargrove is out of the office. (
   5. M'Fadyean Staining of Bacillus anthracis (Owen, Michael P)
   6. Histologists for Cajeput Oil! (Breeden, Sara)
   7. Sectioning of MMA embedded block (Zhang, Renwen)
   8. RE: Re: RED COUNTERSTAINS (Jacqui Detmar)
   9. FW: [Histonet] Histology Supervisor Position NY
  10. rapid freeze containers (Cheri Miller)


Message: 1
Date: Fri, 29 Feb 2008 08:08:57 -0800 (PST)
From: Rene J Buesa 
Subject: Re: [Histonet] Bone Marrow Issues
To: "Metzger, Kenneth" ,
Message-ID: <>
Content-Type: text/plain; charset=iso-8859-1

  BM sections, that are ussually sectioned thinner than others a per
pathologists' request. are more prone to have this artifact ("bubble" or
"empty" nuclei) cause when you DRY the sections in the oven that have
NOT been totally drained.
  If there is some amount of water underneath the section when it goes
into the dying oven, the nuclei contents gets that appearance.
  This artifact has nothing to do with the fixative, is a problem cause
by incompletely dained sections.
  Ren J.

"Metzger, Kenneth"  wrote:
  We are seeing an artifact that causes loss of nuclear detail,
particularly in erythroid cells, giving an appearance of being blown up
with no nuclear detail. It is worse in decaled cores. This appears when
we fix the cores and clot in formalin. We were using Z-5 and did not
have this issue. My Pathologist wants to stick to formalin. None of our
other tissue has this issue. Has anyone had this happen? Any help would
be appreciated.


Ken Metzger HTL(ASCP)
Histology Supervisor
ARUP Laboratories
500 Chipeta way
Salt Lake City, UT 84108
801.583.2787 ext 3101

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Message: 2
Date: Fri, 29 Feb 2008 08:26:58 -0800 (PST)
From: Rene J Buesa 
Subject: Re: [Histonet] frozen slides storage
Message-ID: <>
Content-Type: text/plain; charset=iso-8859-1

I used plastic boxes for 25 slides each.
  Ren J.

"FU,DONGTAO"  wrote:
  Hi, all

I have a question about how to organize frozen slides in 
freezer? We have a lot of slide boxes inside the -80c freezer. It 
is very hard to find which one is which one when we start to do 
IHC staining several weeks later. We want to organize it and put 
all the information in the computer system. Right now I found the 
companies only sell racks for organizing blocks, not for slides(We 
usually use 100 slides box for one submitter). Does anyone have 
any experience on it? How do you organize your unstained slides?

Thank you,

Ann Dongtao Fu MD, Ph.D
Lab Manager
Dept. of Pathology
Lab phone: 352-273-7752
Lab FAX: 352-273-7755
Lab address: D11-50
PO Box: 100275
1600 SW Archer Road
University of Flodrida
Gainesville, FL 32610

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Message: 3
Date: Fri, 29 Feb 2008 08:27:28 -0800 (PST)
From: Rene J Buesa 
Subject: Re: [Histonet] frozen slides storage
Message-ID: <>
Content-Type: text/plain; charset=iso-8859-1

I used plastic boxes for 25 slides each.
  Ren J.

"FU,DONGTAO"  wrote:

Be a better friend, newshound, and know-it-all with Yahoo! Mobile.  Try
it now.


Message: 4
Date: Fri, 29 Feb 2008 10:49:11 -0600
Subject: [Histonet] Susie Hargrove is out of the office.

Content-Type: text/plain; charset=US-ASCII

I will be out of the office starting  02/29/2008 and will not return

I will respond to your message when I return. If  immediate assistance
needed please call 3198.


Message: 5
Date: Fri, 29 Feb 2008 11:50:41 -0500
From: "Owen, Michael P" 
Subject: [Histonet] M'Fadyean Staining of Bacillus anthracis
To: "Histonet" 
Content-Type: text/plain;	charset="us-ascii"

Dear List Members,

Do any of you still perform M'Fadyean (polychrome methylene blue)
staining to visualize the poly-D-glutamic capsule of Bacillus anthracis?
Did you encounter problems with the procedure? 

I am very interested in your experiences with the method including
limitations encountered.

You can contact me off the list to give responses. Thanks in advance.

Michael P. Owen, Regulatory Microbiologist
U.S. FDA Pacific Regional Lab Northwest
22201 23rd Drive SE  Bothell, WA 98021-4421
Phone: 425-483-4865     E-Mail:


Message: 6
Date: Fri, 29 Feb 2008 09:54:21 -0700
From: "Breeden, Sara" 
Subject: [Histonet] Histologists for Cajeput Oil!
Content-Type: text/plain;	charset="us-ascii"

"Histologists for Cajeput Oil"!  There's your bumper sticker.  And if
that doesn't completely befuddle 95% of the population by using two odd
words in one bumper sticker, I don't know what will!


Sally Breeden, HT(ASCP)

NM Dept. of Agriculture

Veterinary Diagnostic Services

PO Box 4700

Albuquerque, NM  87106




Message: 7
Date: Fri, 29 Feb 2008 12:01:06 -0500
From: "Zhang, Renwen" 
Subject: [Histonet] Sectioning of MMA embedded block

Content-Type: text/plain;	charset="us-ascii"

We have some MMA embedded tissue/material blocks. I am looking for a lab
which can make micro sections from the block. The material in the block
is as hard as bone.  


Message: 8
Date: Fri, 29 Feb 2008 12:38:44 -0500
From: Jacqui Detmar 
Subject: RE: [Histonet] Re: RED COUNTERSTAINS
To: Geoff McAuliffe , Robert Richmond
Message-ID: <38F7D55F-A6C0-44AD-9DA1-9A2685448BFC@mimectl>
Content-Type: text/plain; charset="iso-8859-1"; format=flowed

Hi all.  I also bought some brazilin powder (from Anatech) and mixed it
according to directions, and the initial results were disappointing.  I
then prepared an alum brazilin solution (recommended by someone on
Histonet, I think, but I'm at a conference right now and don't have
access to my e-files! ), which is pretty much the same way you
would make alum hematoxylin, except you substitute brazilin for
hematoxylin.  I remember having to use slight heating to get some of the
powder into solution, and even then, not all of the brazilin went into
solution (it falls to the bottom...I guess you could filter it, but I've
never bothered).  

I find that I get the best results if I stain my slides for 20-30
minutes in the alum brazilin, followed by tap-water washes and a final
"blue-ing" in Scott's blueing solution for 30 seconds.  The result is a
nice, raspberry-coloured stain.  The recipe for the brazilin solution is
in the sheet given by the manufacturer.  I love this stain and I no
longer use nuclear fast red.  The only time I've had trouble with it is
with the von Kossa stain.  You *cannot* use brazilin after von
Kossa...the stain goes away for some need to use methyl
green or nuclear fast red.

I think that's about it!  If you have any questions, please feel free to


Jacqui Detmar, Post-doctoral Fellow
Samuel Lunenfeld Research Institute, room 876
Mount Sinai Hospital
600 University Avenue,
Toronto, ON, Canada
M5G 1X5

Tel:       416-586-4800 x2451/x2290
Fax:      416-586-8588

From: Geoff McAuliffe
Sent: Fri 2/29/2008 10:25 AM
To: Robert Richmond
Subject: Re: [Histonet] Re: RED COUNTERSTAINS

Hi Bob:

    I bought some Brazilliant recently and mixed it according to the 
directions provided. The results were dissipointing, red-orange nuclei, 
not very intense. The tissue was rabbit kidney fixed in 
formalin-alcohol-acetic. An alum hematoxylin on the same tissue looks


Robert Richmond wrote:
> Diana McCaig asks about the nuclear counterstains nuclear fast red and
> neutral red.
> Does anyone on this list have any experience with Anatech's
> "Brazilliant", their trade name for alum brazilin, closely related to
> alum hematoxylin, but red instead of purple? This looks to me like a
> very logical red nuclear stain, and I'd certainly like to see it in
> action if it were possible for me to obtain it (remember that hospital
> pathology services are not usually permitted to order from small
> companies like Anatech and the Davidson marking ink people).
> As everybody on this list ought to know, hematoxylin is a dye
> extracted from the logwood tree (Haematoxylum campechianum), with an
> aluminum mordant. (There is no satisfactory synthetic substitute.)
> Brazilin is structurally very similar, but with an alum mordant it is
> red rather than purple. Brazilin is extracted from the closely related
> brazil woods, Caesalpinia echinata or C. sappan.
> One would expect this red dye to have the same staining specificity as
> hematoxylin, and it should not wash out in aqueous mounting media.
> (I have no connection with Anatech.)
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
> _______________________________________________
> Histonet mailing list

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583

Histonet mailing list


Message: 9
Date: Fri, 29 Feb 2008 17:41:52 +0000
Subject: FW: [Histonet] Histology Supervisor Position NY
Content-Type: text/plain

-------------- Forwarded Message: -------------- 
Subject: [Histonet] Histology Supervisor Position NY 
Date: Wed, 27 Feb 2008 16:38:58 +0000 

I have a position for a Histology Supervisor available at an established
group located in New City, NY, Rockland County. Candidate should have
knowledge to set up and maintain a state of the art facility. Knowledge
grossing, processing and routine histology of gastric biopsies required.

$30.00/hr plus benefits. 
Qualified candidates can email me at or call me
at 732 
$500.00 for a successful referals committed for at least 90 days. 

Marilynn Weber H.T. (ASCP) QIHC 
Histonet mailing list 


Message: 10
Date: Fri, 29 Feb 2008 11:59:48 -0600
From: "Cheri Miller" 
Subject: [Histonet] rapid freeze containers
Message-ID: <000001c87afc$df974840$3d02a8c0@plab.local>
Content-Type: text/plain;	charset="us-ascii"

How is everyone disposing of their rapid freeze aerosol cans when empty?
is considered hazardous waste here in Omaha, not bio hazard but
hazardous in
the terms that the can is under pressure. We can purchase a can
for 1000.00 but that seems steep.


Cheryl Miller HT (ASCP)

Histology Supervisor

Physicians Laboratory,P.C.

Omaha, Ne. 

402 738 5052


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