Sonny:
Sudan III is prepared in 70% ethanol, but its solubility is a bout 0.2 g/100 mL so you will end with an oversaturated solution = with undissolved dye in the bottom of the flask.
  René J.

Sonny Duong  wrote:
  Hi all!

I am attempting to perform fecal fat microscopy with Sudan 
III stain. My protocol requires me to have 1% Sudan III 
dye, and my question is, 1% in what? The dye is insoluble 
in water, so what should I dissolve the stain in? Ehtanol? 
Chloroform??

Thanks,

Sonny Duong

Carla Green Lab
University of Virginia


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