From:Geoff McAuliffe

Hi Bob:

    I bought some Brazilliant recently and mixed it according to the 
directions provided. The results were dissipointing, red-orange nuclei, 
not very intense. The tissue was rabbit kidney fixed in 
formalin-alcohol-acetic. An alum hematoxylin on the same tissue looks fine.


Robert Richmond wrote:
> Diana McCaig asks about the nuclear counterstains nuclear fast red and
> neutral red.
> Does anyone on this list have any experience with Anatech's
> "Brazilliant", their trade name for alum brazilin, closely related to
> alum hematoxylin, but red instead of purple? This looks to me like a
> very logical red nuclear stain, and I'd certainly like to see it in
> action if it were possible for me to obtain it (remember that hospital
> pathology services are not usually permitted to order from small
> companies like Anatech and the Davidson marking ink people).
> As everybody on this list ought to know, hematoxylin is a dye
> extracted from the logwood tree (Haematoxylum campechianum), with an
> aluminum mordant. (There is no satisfactory synthetic substitute.)
> Brazilin is structurally very similar, but with an alum mordant it is
> red rather than purple. Brazilin is extracted from the closely related
> brazil woods, Caesalpinia echinata or C. sappan.
> One would expect this red dye to have the same staining specificity as
> hematoxylin, and it should not wash out in aqueous mounting media.
> (I have no connection with Anatech.)
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
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> Histonet mailing list

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583

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