Cheryl,
I do a lot of rodent tissue here and even though I process it myself
occasionally there are some that are pretty dried out and need soaking. A few
months ago there was a discussion on histonet about putting glycerin in the
water that you soak your faced off blocks in. I tried this and it works
wonderfully. We don't measure out the glycerin although I think if you go back
in the histonet archives you will find the actual "recipe". We just put a small
amount of glycerin into a 500 ml bottle and fill
it up with DI water - shake and
soak.
And I have noticed that my fingertips are now softer too!
Andi Grantham
At 07:42 AM 2/21/2008, Rene J Buesa wrote:
>Cheryl:
> Rodent tissues are inherently lower in fat
> and with less water in their fat tissue and
> this is a "dangerous" combination when
> processing because, as you point out, the
> tissues you received are overprocessed so you
> will have ptoblems sectioning, extending the
> sections and holding them to your slides.
> Try ALL the following steps:
> 1- prepare a solution of 0.2% (v/v) of fabric
> softener and prepare a frozen block with it.
> This frozen 0.2% fabric softerner will be used
> to cold the blocks. Face them off and place
> them face down over the frozen softener covered
> with paper towels in a way that the blocks are
> going to be in contact with the softener for at
> least 30 minutes before trying to section.
> 2- increase the temperature of your water
> bath at 50-52ºC, close to the paraffin melting point WITHOUT reaching it.
> 3- add to your water bath 0.25 mL of liquid
> detergent (but NOT dish washer). This step, and
> the previous one are designed to lower the
> water surface tension and facilitate the sections expansion.
> 4- add 1 mL of Elmer's (white) glue to the
> water bath. The water will be slightly whitish
> but this weak glue solution will help to hold the sections to the slides.
>
> When you get to the area in the block that
> you want to section, cool it with a ice cube
> wrapped in a gauze and take the sections SLOWLY.
>
> This combinaiton should help you, but all the steps together.
> Good luck with your rodents' blocks!
>René J.
>
>Cheryl wrote:
>
>
>
>
>
>
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: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212) P.O. Box 245044 :
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