Make sure you rinse well after the bluing with distilled water (you don't
want to bring the pH up on the eosin from carry over of hard tap water or
bluing reagent). Go into alcohol before eosin and don't do a water rinse
after eosin. Make sure your first alcohol after eosin is 95% and fresh.
On Wed, Feb 27, 2008 at 9:06 AM, Martin, Erin wrote:
> Hi everyone,
> I am having a problem with my eosin in the H&E. My pathologists say that
> it is dull and the RBCs are too red rather than orange. Interestingly, when
> I take out the hematoxylin (Richard Allen Harris), acid acohol (Richard
> Allen Clarifier) and bluing (Richard Allen Bluing) the eosin is nice and
> bright. Our tap water for the rinses is pH 6.8, and we use a Sakura Prisma
> stainer. Any ideas?
> Thank in advance!
> Erin Martin
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