Re: [Histonet] Calretinin

From:Rene J Buesa

Your problem is not with calretinin but with a poorly infiltrated tissue sectioned for the test.
  If you can process another piece of the tissue, or add a coat of Elmer's glue on a slide, let dry and use it to pick up new sections of your case.
  René J.

"Senn, Amy R"  wrote:
  Hi Histoland....

We are having some problems with our Calret stains.

Our patient tissue keeps floating off & we are not sure what we're doing
wrong (if anything!)

We do use charged slides.

We float the tissue out in a separate water bath with pure DI water.

We allow the slides to stand upright for 30 minutes to air dry, and then
put them in a 62 degree oven for another 30 minutes.

We are careful not to over-handle the slides at anytime, and we make
sure our hands are clean and lotion free.

Sometimes, for weeks and weeks, the tissue is right where we put it when
it comes off the stainer. Other times, for weeks and weeks, it's gone.
And it's only the patient--the control is where's it's supposed to be.

Is there something we're missing? Something else we can try??

Thanks so much for all your help!!

Amy S.

Histotech, Histology Laboratory

Holy Spirit Hospital

503 N. 21st Street

Camp Hill, PA 17011

(717) 763-2124

Cell: (724) 494-2237

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