If the problem was absolute alcohol between the xylenes and the paraffin, the tissue should be rerun through fresh xylenes to remove both the poorly infiltrated paraffin and any remaining alcohol. Then through fresh xylenes to reinfiltrate the tissue properly. I can't guarantee there won't be any negative effects, but this is about all you can do at this point, and hopefully the tissues will section a lot better after this treatment. If you have vacuum available on your processor, use it on all stations during this rerun.
> From: email@example.com on behalf of Laurie Colbert
> Sent: Wednesday, February 20, 2008 1:15 PM
> To: firstname.lastname@example.org
> Subject: [Histonet] HELP!! With tissue processing
> I'm hoping someone can give me some advice on fixing our biopsy
> specimens that were "fried" last night during processing.
> Someone put a 100% alcohol where the last xylene (before the paraffin)
> should have been. The problem was discovered during embedding. At this
> point, someone switched out the cleaning alcohol bottle with xylene, and
> put the tissue through two changes of xylene on the clean cycle. I
> think this is what fried the tissues. The tissue is very dry and
> unreadable, and needless to say, the pathologists are livid! I would
> appreciate any help you can give.
> Laurie Colbert
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