RE: [Histonet] FISH cytology fixatives

From:"Tony Henwood"

If you are looking for DNA translocations, 95% ethanol works well.


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
[] On Behalf Of Kim
Sent: Wednesday, 27 February 2008 11:42 PM
To: Histonet
Subject: [Histonet] FISH cytology fixatives

Hi Everyone,
  I am working up some FISH staining protocols for cytology (cell
culture) slides (eventually to be OCT and FFPE tissues, but I need to
walk before I can run).
  Most of the journals that I have read recommend using a fixative
containing methanol and acetic acid (3:1).  The DAKO probe I am using
calls for the use of 3.7% fomaldehyde.  I am wondering why acetone (or
acetone/ethanol) is not the routine fixative for such a procedure.  Can
someone let me know why to choose one fixative over another and what the
merits of methanol/glacial acetic acid would be?
  Thanks in advance,

Kim Merriam, MA, HT(ASCP)
Cambridge, MA
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