If you are looking for DNA translocations, 95% ethanol works well.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim
Merriam
Sent: Wednesday, 27 February 2008 11:42 PM
To: Histonet
Subject: [Histonet] FISH cytology fixatives
Hi Everyone,
I am working up some FISH staining protocols for cytology (cell
culture) slides (eventually to be OCT and FFPE tissues, but I need to
walk before I can run).
Most of the journals that I have read recommend using a fixative
containing methanol and acetic acid (3:1). The DAKO probe I am using
calls for the use of 3.7% fomaldehyde. I am wondering why acetone (or
acetone/ethanol) is not the routine fixative for such a procedure. Can
someone let me know why to choose one fixative over another and what the
merits of methanol/glacial acetic acid would be?
Thanks in advance,
Kim
Kim Merriam, MA, HT(ASCP)
Cambridge, MA
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