What eosin are you using?

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin,
Erin
Sent: Wednesday, February 27, 2008 11:07 AM
To: histonet
Subject: [Histonet] Dull eosin?


Hi everyone, 
 
I am having a problem with my eosin in the H&E.  My pathologists say
that it is dull and the RBCs are too red rather than orange.
Interestingly, when I take out the hematoxylin (Richard Allen Harris),
acid acohol (Richard Allen Clarifier) and bluing (Richard Allen Bluing)
the eosin is nice and bright. Our tap water for the rinses is pH 6.8,
and we use a Sakura Prisma stainer.  Any ideas?
 
Thank in advance!
 
Erin Martin
 


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