RE: [Histonet] Buffer for ISH

From:"Liz Chlipala"


I was looking for one too, but I could not locate one.  We prepared our
own.  We buy alot of our probes from Genedetect and they were telling me
that in the future they may provide an hybidization buffer.  I use
theirs, it takes about 2 days to make because of the high concentration
of dextran sulfate.  Teri Johnson sent me one that looked a bit simpler.
I have a SOP for the one I use if you are interested.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
Ship to Address:
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
[] On Behalf Of Richard
Sent: Thursday, February 21, 2008 10:00 AM
To: Histonet
Subject: [Histonet] Buffer for ISH

I am posting this question for a friend that works in veterinary

"is there a commercially available hybridization buffer for ISH that can
be purchased separately (or as part of a kit)?"



Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

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