RE: [Histonet] Ammonia water and H+E stain

From:"Kemlo Rogerson"

"I recently asked this on the histonet, and would like to get some more
on it.
Could soaking faced paraffin blocks in ammonia water too long negatively

affect the H+E stain?  I
Could ammonia change the acid/base charges or pH of different tissue 
components that could negatively effect the charges necessary for heme
staining, or 
Thanks for letting me "rerun" this question"

If you leave something in anything too long then something is likely to
alter, but why do it? I suppose that opens the wound again of you
Americans poorly fixing and processing stuff so that you resort to
cutting frozen sections at room temperature!!! 
Kemlo Rogerson        
Pathology Manager
DD   01934 647057 or extension 3311
Mob 07749 754194; Pager 07659 597107;

Don't be afraid to take a big step when one is indicated. You can't
cross a chasm in two small jumps. --Buckminster Fuller 

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