Can anyone suggest which antibodies to use for RCC and Caldesmon? We have tried Dako's RCC and want to try another company. The Caldesmon is new for us, so any suggestion is greatly appreciated.
Thank you for your time!
Yudi Bolig
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu
Sent: Sunday, February 17, 2008 1:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 51, Issue 26
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Today's Topics:
1. Iron control (Cheryl Crowder)
2. Help with FISH on urine samples... (Jennifer Easterling)
3. Re: Help with FISH on urine samples... (Rene J Buesa)
4. RE: GMS and Bouin's fixation (Lee & Peggy Wenk)
5. AW: [Histonet] Help with FISH on urine samples... (Gudrun Lang)
6. Scabies (Rathborne, Toni)
----------------------------------------------------------------------
Message: 1
Date: Sat, 16 Feb 2008 12:40:02 -0600
From: "Cheryl Crowder"
Subject: [Histonet] Iron control
To: "histonet@lists.utsouthwestern.edu"
Message-ID:
Content-Type: text/plain; charset="us-ascii"
If your looking for a gorgeous iron positive control try a rabbit liver.
They have free iron and stain beautifully with all iron stains.
Cheryl
Cheryl Crowder, BA, HTL(ASCP)
Chief Technologist
Anatomic Pathology
Department of Pathobiological Sciences
School of Veterinary Medicine
Louisiana State University
Skip Bertman Drive
Baton Rouge, LA 70803
225-578-9734
FAX: 225-578-9720
------------------------------
Message: 2
Date: Sat, 16 Feb 2008 11:46:40 -0800 (PST)
From: Jennifer Easterling
Subject: [Histonet] Help with FISH on urine samples...
To: histonet@lists.utsouthwestern.edu
Message-ID: <561398.92942.qm@web39814.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hello everyone, I am new to this list and I have found it very helpful. Hopefully, someone can help me with my problem...There is a lab in my area wanting to start FISH on urine samples for bladder cancer. They are in the very beginning stages and have no policy or procedure manual in place. If anyone has any information on either of these two things, any help would be greatly appreciated.
Thanks in advance,
Jennifer
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Message: 3
Date: Sat, 16 Feb 2008 13:00:04 -0800 (PST)
From: Rene J Buesa
Subject: Re: [Histonet] Help with FISH on urine samples...
To: Jennifer Easterling ,
histonet@lists.utsouthwestern.edu
Message-ID: <96706.87445.qm@web61211.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Jennifer:
Try to contact a cytotechnologist in your area and s/he will tell you how to prepare cytospins from urine. With the cytospins you can prepare slides to treat with FISH for the cancer probes you want to use.
Ren? J.
Jennifer Easterling wrote:
Hello everyone, I am new to this list and I have found it very helpful. Hopefully, someone can help me with my problem...There is a lab in my area wanting to start FISH on urine samples for bladder cancer. They are in the very beginning stages and have no policy or procedure manual in place. If anyone has any information on either of these two things, any help would be greatly appreciated.
Thanks in advance,
Jennifer
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Message: 4
Date: Sat, 16 Feb 2008 18:43:27 -0500
From: "Lee & Peggy Wenk"
Subject: RE: [Histonet] GMS and Bouin's fixation
To: "'Paul Bradbury'" , ,
"'HistoNet Server'"
Message-ID: <000701c870f5$baa5b6e0$0202a8c0@HPPav2>
Content-Type: text/plain; charset="us-ascii"
The reasoning sounds right to me.
I might suggest using 0.5% periodic acid (such as in the PAS stain) for 5-10 minutes as the oxidizer for the GMS stain, instead of the chromic acid.
Since the picric acid has started the carbohydrate oxidation, and the chromic acid is then overoxidizing the remaining carbohydrates, then using a mild oxidizer such as periodic acid might do the trick. Usually, periodic acid is NOT used in the GMS stain, as not enough background is overoxidized.
The PASM/Jones stain used to demonstrate basement membranes in kidney biopsies is the same silver methenamine solution as in the GMS, but periodic acid is used instead of chromic acid. Our kidney biopsies are fixed in DB, which is an alcoholic-Bouins, which does contain picric acid. But the PASM/Jones stain continues to demonstrate basement membrane. If a GMS is done on the kidney biopsy, the basement membrane is not demonstrated.
So I think using 0.5% periodic acid for 5-10 minutes, rinsing in d. water, and then going into the GMS methenamine silver solution should work.
Peggy A. Wenk, HTL(ASC)SLS
William Beaumont Hospital
Royal Oak, MI 48073
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paul Bradbury
Sent: Saturday, February 16, 2008 2:53 AM
To: alineumann@aol.com; HistoNet Server
Subject: [Histonet] GMS and Bouin's fixation
Hi Alice,
Logically, there may be a conflict between fixing tissues in Bouin's and successful demonstration of fungi using a methemamine silver technique.
This is my reasoning:
Bouin has long been the recommended fixative for glycogen and proteoglygans which are best demonstrated by the PAS reaction. In the PAS, carbohydrate groups are oxidised by periodic acid to form aldehydes, which in turn react with Schiff reagent to produce the magenta colour. Periodic acid, as a 1% solution at room temperature, will oxidize these groups only as far as the aldehyde stage.
The methenamine silver is essentially a modification of that same concept, but using chromic acid and an unstable silver solution in place of the periodic acid and Schiff reagent. In the methenamine silver technique, the carbohydrates in the capsule surrounding the fungal elements are oxidized to form aldehydes which reduce the silver solution to produce visible deposits of silver. Prolonged treatment with chromic acid will over-oxidize the carbohydrates to carboxyl groups which are non-reactive with the silver solution.
There may well be a reaction between the picric acid in Bouin's fixative and carbohydrates. Picric acid is a potent oxidizer and may begin the oxidation of the carbohydrate groups in the fungal capsule. When the sections are further oxidized by chromic acid, the fungal walls become over-oxidized and form non-reactive carboxyl groups.
It may be worth trying a much shorter chromic acid treatment on Bouin's fixed tissues to see if this will leave the carbohydrates in the fungi at the aldehyde stage.
Most methenamine silver techniques suggest a 60 minutes treatment in 5% chromic acid at room temperature. In the case of Bouin-fixed tissues, I would suggest running a trial using a range of chromic acid times from 10-40 minutes to see if the fungi are still reactive with one of the shorter oxidation times.
I would be very interested to know if this solves your problem.
Paul Bradbury
Kamloops, BC
Canada
alineumann@aol.com wrote:
> Hello all, has anyone ever experienced Bouin's fixed tissue?preventing
> the methenamine from staining fungus?? Thank you,
>
>
> Alice Neumann MD
> Western Wyoming Pathology
> Jackson, WY 83001
>
> Cell: 307-413-4042
> Work: 307-733-6418
> Home: 307-734-4410
> Fax: 307-734-0885
>
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Message: 5
Date: Sun, 17 Feb 2008 12:18:03 +0100
From: "Gudrun Lang"
Subject: AW: [Histonet] Help with FISH on urine samples...
To: "'Jennifer Easterling'"
Cc: histonet@lists.utsouthwestern.edu
Message-ID: <001e01c87156$c3c06070$eeeea8c0@dielangs.at>
Content-Type: text/plain; charset="iso-8859-1"
I think, this site could help you.
http://www.urovysion.com/proceduraloverview_358.aspx
Gudrun Lang
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754
-----Urspr?ngliche Nachricht-----
Von: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Jennifer Easterling
Gesendet: Samstag, 16. Februar 2008 20:47
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Help with FISH on urine samples...
Hello everyone, I am new to this list and I have found it very helpful.
Hopefully, someone can help me with my problem...There is a lab in my area wanting to start FISH on urine samples for bladder cancer. They are in the very beginning stages and have no policy or procedure manual in place. If anyone has any information on either of these two things, any help would be greatly appreciated.
Thanks in advance,
Jennifer
---------------------------------
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------------------------------
Message: 6
Date: Sun, 17 Feb 2008 12:24:28 -0500
From: "Rathborne, Toni"
Subject: [Histonet] Scabies
To:
Message-ID:
Content-Type: text/plain; charset="utf-8"
Hi all!
I have a question about the proper way to process/prepare skin scrapings for scabies. We have had a few cases in the last year, and want to be sure that they are being submitted properly. Also, does anyone know where we might purchase a positive slide/picture to identify the eggs. With something that occurs so infrequently, the pathologist's would like to have a reference so that they know what they are looking for.
Thanks,
Toni
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End of Histonet Digest, Vol 51, Issue 26
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