[Histonet] RE: Histonet Digest, Vol 51, Issue 40

From:"Jacobs, Genie"



Kappa/lambda CISH geniejacobs)
We are starting up Kappa lambda cish and would like any info on protocol and vendors  Does anyone have any experience using the Histosonda probe made by CENBIMO? thanks
 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu
Sent: Wednesday, February 27, 2008 12:03 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 51, Issue 40

Send Histonet mailing list submissions to
	histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
	http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
	histonet-request@lists.utsouthwestern.edu

You can reach the person managing the list at
	histonet-owner@lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..."


Today's Topics:

   1. FISH cytology fixatives (Kim Merriam)
   2. Re: FISH cytology fixatives (godsgalnow@aol.com)
   3. Re: Fixing crab blood smear (Rene J Buesa)
   4. RE: Night differential (Cheri Miller)
   5. goldners trichrome (Simoskevitz@Osteotech.com)
   6. Lyme Disease IHC/PCR (histosprv06@aol.com)
   7. Histology Supervisor Position NY (rmweber113@comcast.net)
   8. reference point slides. (Garry Ashton)
   9. reference point slides. (Garry Ashton)
  10. Dull eosin? (Martin, Erin)
  11. RE: Dull eosin? (Mike Pence)
  12. RE: Dull eosin? (Bartlett, Jeanine (CDC/CCID/NCZVED))
  13. FW: Region III Hosted by Georgia Society for	Histotechnology
      (Shirley Powell)


----------------------------------------------------------------------

Message: 1
Date: Wed, 27 Feb 2008 04:42:25 -0800 (PST)
From: Kim Merriam 
Subject: [Histonet] FISH cytology fixatives
To: Histonet 
Message-ID: <668890.34731.qm@web50312.mail.re2.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hi Everyone,
   
  I am working up some FISH staining protocols for cytology (cell culture) slides (eventually to be OCT and FFPE tissues, but I need to walk before I can run).
   
  Most of the journals that I have read recommend using a fixative containing methanol and acetic acid (3:1).  The DAKO probe I am using calls for the use of 3.7% fomaldehyde.  I am wondering why acetone (or acetone/ethanol) is not the routine fixative for such a procedure.  Can someone let me know why to choose one fixative over another and what the merits of methanol/glacial acetic acid would be?
   
  Thanks in advance,
  Kim


Kim Merriam, MA, HT(ASCP)
Cambridge, MA
       
---------------------------------
Never miss a thing.   Make Yahoo your homepage.

------------------------------

Message: 2
Date: Wed, 27 Feb 2008 08:24:11 -0500
From: godsgalnow@aol.com
Subject: Re: [Histonet] FISH cytology fixatives
To: kmerriam2003@yahoo.com, histonet@lists.utsouthwestern.edu
Message-ID: <8CA475B2C9387AD-804-4E7@FWM-M08.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"


In Cytological preparation, one would use an acetic acid in the fixative to lyse the RBC's, which could ultimaetly interfere with the diagnosis.? Journal articles are great to help you research and give you a starting point for what it is you want to do, that is where I started.? But when you start doing the procedure, or playing with until you get it to work, you always need to start with the suggestions of the manufacturer of the probe.? That is how they validated it and it is known to work under those conditions.? At that point you can play with fixatives, if you need to.

A lot of FISH testing requires post fixation anyway.? For, instance, the tissue comes in formalin (or whatever), but after you de-wax the slides you have to begin the pre-treatment, an most of the time there is a step in there that involves placing the slides in formalin (or whatever).



Roxanne


-----Original Message-----
From: Kim Merriam 
To: Histonet 
Sent: Wed, 27 Feb 2008 7:42 am
Subject: [Histonet] FISH cytology fixatives




Hi Everyone,
   
  I am working up some FISH staining protocols for cytology (cell culture) slides (eventually to be OCT and FFPE tissues, but I need to walk before I can run).
   
  Most of the journals that I have read recommend using a fixative containing methanol and acetic acid (3:1).  The DAKO probe I am using calls for the use of 3.7% fomaldehyde.  I am wondering why acetone (or acetone/ethanol) is not the routine fixative for such a procedure.  Can someone let me know why to choose one fixative over another and what the merits of methanol/glacial acetic acid would be?
   
  Thanks in advance,
  Kim


Kim Merriam, MA, HT(ASCP)
Cambridge, MA
       
---------------------------------
Never miss a thing.   Make Yahoo your homepage.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 3
Date: Wed, 27 Feb 2008 05:37:27 -0800 (PST)
From: Rene J Buesa 
Subject: Re: [Histonet] Fixing crab blood smear
To: "Cheah, Lit Chien" ,
	histonet@lists.utsouthwestern.edu
Message-ID: <927292.99610.qm@web61223.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Your procedure is standard, even for crab or spiny lobster for that matter (even when it really is hemolymph). They should be OK
  Do you know Dr. Phillips (working with spiny lobsters? I have lost contact with him since many years ago).
René J. Buesa

"Cheah, Lit Chien"  wrote:
  Dear All, 

I have been ask is there a batter way to fix crab blood smear before the slides travel 800 kilometers for us to do Giemsa Stain, appart from the normal air dry then fix in methanol and shipment.

Big Thanks in advance from the largest State in Australia!


Mr. Lit Chien CHEAH
Histopathology Supervisor
BSC (Medical Science)

Animal Health Laboratories
3 Baron-Hay Court
South Perth, 6155 WA.













This e-mail and files transmitted with it are privileged and confidential information intended for the use of the addressee. The confidentiality and/or privilege in this e-mail is not waived, lost or destroyed if it has been transmitted to you in error. If you received this e-mail in error you must
(a) not disseminate, copy or take any action in reliance on it;
(b) please notify the Department of Agriculture and Food, WA immediately by return e-mail to the sender;
(c) please delete the original e-mail.

This email has been successfully scanned by McAfee Anti-Virus software.
Department of Agriculture and Food WA
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




       
---------------------------------
Never miss a thing.   Make Yahoo your homepage.

------------------------------

Message: 4
Date: Wed, 27 Feb 2008 08:22:25 -0600
From: "Cheri Miller" 
Subject: RE: [Histonet] Night differential
To: "'Cindy DuBois'" ,
	
Message-ID: <001501c8794c$2cd6f720$3d02a8c0@plab.local>
Content-Type: text/plain;	charset="us-ascii"

Where I used to work it was 15% of your hourly wage for any hours worked from 1100pm to 6am

Cheryl Miller HT (ASCP)
Histology Supervisor
Physicians Laboratory,P.C.
Omaha, Ne. 
402 738 5052

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy DuBois
Sent: Tuesday, February 26, 2008 10:12 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Night differential

Are there any techs who get a night differential?  If so, how much?
We currently come to work @ 3:30 am.  We are now being asked to come in at 1:30.  I would like to ask for a night differential, but need to know how much to ask for.

Thanks,
Cindy
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message.  If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information.  Any dissemination, distribution, or copying of this e-mail is strictly prohibited.  If you have received this message in error, please notify the sender immediately and delete this email from your system.



PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message.  If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information.  Any dissemination, distribution, or copying of this e-mail is strictly prohibited.  If you have received this message in error, please notify the sender immediately and delete this email from your system.





------------------------------

Message: 5
Date: Wed, 27 Feb 2008 10:19:52 -0500
From: Simoskevitz@Osteotech.com
Subject: [Histonet] goldners trichrome
To: histonet@lists.utsouthwestern.edu
Message-ID:
	
	
Content-Type: text/plain; charset=US-ASCII


Barbara,

I was asked to do a Goldners Trichrome in Methymethacylate embedded bone =2E
Do you have a good protocol for this or do you know where I could find one.

Thank you for any help you can give me.

Ricki Simoskevitz
Osteotech Inc.
Eatontown, NJ 07724
(732) 542-2800 X6328




------------------------------

Message: 6
Date: Wed, 27 Feb 2008 10:29:35 -0500
From: histosprv06@aol.com
Subject: [Histonet] Lyme Disease IHC/PCR
To: histonet@lists.utsouthwestern.edu
Message-ID: <8CA476CB1044DEB-80C-AE3@webmail-da16.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"


Good Morning everyone, no better place to come for advice than the histonet! Does anyone know of a facility where?we can send a processed skin block for Borrelia Burgdorferi via IHC and PCR as well as Erlichiosis via PCR? We are in Florida and are not having much luck finding?a place?that offers this testing.?

We would greatly appreciate any knowledge you can provide.

Thanks in advance.

?
Kari Zajic, HT


------------------------------

Message: 7
Date: Wed, 27 Feb 2008 16:36:07 +0000
From: rmweber113@comcast.net
Subject: [Histonet] Histology Supervisor Position NY
To: histonet@lists.utsouthwestern.edu
Message-ID:
	<022720081636.3418.47C591770005560100000D5A2215575114CCCECE9D0A0D0A99039D@comcast.net>
	
Content-Type: text/plain

I have a position for a Histology Supervisor  available at an established GI group located in New City, NY, Rockland County.  Candidate should have the knowledge to set up and maintain a state of the art facility.  Knowledge of grossing, processing and routine histology of gastric biopsies required.  $30.00/hr plus benefits.
Qualified candidates can email me at rmweber113@comcast.net or call me at 732 814-1170 $500.00 for a successful referals committed for  at least 90 days.

Marilynn Weber H.T. (ASCP) QIHC
Twincrest

------------------------------

Message: 8
Date: Wed, 27 Feb 2008 16:39:18 -0000
From: "Garry Ashton" 
Subject: [Histonet] reference point slides.
To: ,	"histonet"
	
Message-ID:
	
Content-Type: text/plain;	charset="us-ascii"

Hi everyone,
Has anybody used or had any experience of microscope slides with some type of reference point on them?
Thanks in advance.
Garry
 
 
 
PICR
UK
 
--------------------------------------------------------

 
This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the University of Manchester. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible.
 


------------------------------

Message: 9
Date: Wed, 27 Feb 2008 16:39:18 -0000
From: "Garry Ashton" 
Subject: [Histonet] reference point slides.
To: ,	"histonet"
	
Message-ID:
	
Content-Type: text/plain;	charset="us-ascii"

Hi everyone,
Has anybody used or had any experience of microscope slides with some type of reference point on them?
Thanks in advance.
Garry
 
 
 
PICR
UK
 
--------------------------------------------------------

 
This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the University of Manchester. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible.
 


------------------------------

Message: 10
Date: Wed, 27 Feb 2008 09:06:53 -0800
From: "Martin, Erin" 
Subject: [Histonet] Dull eosin?
To: "histonet" 
Message-ID:
	
Content-Type: text/plain; charset=iso-8859-1

Hi everyone, 
 
I am having a problem with my eosin in the H&E.  My pathologists say that it is dull and the RBCs are too red rather than orange.  Interestingly, when I take out the hematoxylin (Richard Allen Harris), acid acohol (Richard Allen Clarifier) and bluing (Richard Allen Bluing) the eosin is nice and bright. Our tap water for the rinses is pH 6.8, and we use a Sakura Prisma stainer.  Any ideas?
 
Thank in advance!
 
Erin Martin
 




------------------------------

Message: 11
Date: Wed, 27 Feb 2008 11:13:35 -0600
From: "Mike Pence" 
Subject: RE: [Histonet] Dull eosin?
To: "Martin, Erin" ,	"histonet"
	
Message-ID:
	<661949901A768E4F9CC16D8AF8F2838C017A373E@IS-E2K3.grhs.net>
Content-Type: text/plain;	charset="us-ascii"

What eosin are you using?

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Erin
Sent: Wednesday, February 27, 2008 11:07 AM
To: histonet
Subject: [Histonet] Dull eosin?


Hi everyone, 
 
I am having a problem with my eosin in the H&E.  My pathologists say that it is dull and the RBCs are too red rather than orange.
Interestingly, when I take out the hematoxylin (Richard Allen Harris), acid acohol (Richard Allen Clarifier) and bluing (Richard Allen Bluing) the eosin is nice and bright. Our tap water for the rinses is pH 6.8, and we use a Sakura Prisma stainer.  Any ideas?
 
Thank in advance!
 
Erin Martin
 


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





------------------------------

Message: 12
Date: Wed, 27 Feb 2008 12:22:28 -0500
From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" 
Subject: RE: [Histonet] Dull eosin?
To: "Martin, Erin" ,	histonet
	
Message-ID:
	<34BB307EFC9A65429BBB49E330675F72045E25FA@LTA3VS003.ees.hhs.gov>
Content-Type: text/plain; charset=us-ascii

I assume you are using the Richard Allan eosin Y alcoholic? 


Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartlett@cdc.hhs.gov


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin,
Erin
Sent: Wednesday, February 27, 2008 12:07 PM
To: histonet
Subject: [Histonet] Dull eosin?

Hi everyone, 
 
I am having a problem with my eosin in the H&E.  My pathologists say
that it is dull and the RBCs are too red rather than orange.
Interestingly, when I take out the hematoxylin (Richard Allen Harris),
acid acohol (Richard Allen Clarifier) and bluing (Richard Allen Bluing)
the eosin is nice and bright. Our tap water for the rinses is pH 6.8,
and we use a Sakura Prisma stainer.  Any ideas?
 
Thank in advance!
 
Erin Martin
 


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





------------------------------

Message: 13
Date: Wed, 27 Feb 2008 12:25:09 -0500
From: Shirley Powell 
Subject: [Histonet] FW: Region III Hosted by Georgia Society for
	Histotechnology
To: histonet@lists.utsouthwestern.edu
Message-ID: <01MRSH6CO2WQ001E0L@Macon2.Mercer.edu>
Content-Type: text/plain; charset=us-ascii

 
I sent this out yesterday, but I never received it myself so this is just to
make sure you guys get it.

-----Original Message-----
From: Shirley Powell [mailto:powell_sa@mercer.edu] 
Sent: Tuesday, February 26, 2008 4:48 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Region III Hosted by Georgia Society for Histotechnology

Hi Guys, 

We sent out over 1000 programs for the Region III meeting to be held in
Atlanta Georgia April 3-5, 2008 at the Westin Peachtree Plaza Hotel.  But
there may be some members that we missed and feel it necessary to post a
reminder for all who wish to attend our meeting.  

The deadline for the hotel reservations for the discounted rate is March
3rd.  That rate is $129 single, double, triple or quad.  The phone number to
the Westin is 1-404-659-1400 and please state that you are attending the
GSH/Region III meeting.  

The revised program can be downloaded from our website at
www.histosearch.com/gsh.  Click on the symposium link to get a PDF file of
the program.  Vendors have a link to the registration form to exhibit at the
meeting on the same page.  If you have questions Chris Coley, GSH Exhibit
Liaison, has contact information is on that form

If anyone has any questions please feel to contact me at this email address.


Come on down to Georgia and experience some warmer weather.  

Shirley Powell
GSH Secretary/Registrar




------------------------------

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

End of Histonet Digest, Vol 51, Issue 40
****************************************




The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law.  If you are not the intended recipient, you are prohibited from copying, distributing, or using the information.  Please contact the sender immediately by return e-mail and delete the original message from your system.

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




<< Previous Message | Next Message >>