Cheryl Miller HT (ASCP)
Histology Supervisor
Physicians Laboratory,P.C.
Omaha, Ne.
402 738 5052
_____
From: Cheri Miller [mailto:cmiller@physlab.com]
Sent: Thursday, February 28, 2008 11:31 AM
To: histonet-bounces@lists.utsouthwestern.edu
Subject: FW: under processed tissue
Cheryl Miller HT (ASCP)
Histology Supervisor
Physicians Laboratory,P.C.
Omaha, Ne.
402 738 5052
_____
From: Cheri Miller [mailto:cmiller@physlab.com]
Sent: Thursday, February 28, 2008 11:01 AM
Cc: 'mfokol@sakuraus.com'
Subject: under processed tissue
Hi, I hope someone out there can help me. I am having issues with
under-processed tissue. A few weeks ago we had power outages and our VIP
2000 battery backup didn't work. We have since fixed this problem and
everything on the VIP is working as before. My problem is I had to set up my
processing programs again.( the programs were set prior to my employment) I
suspect my times are not the same in each station as before and are causing
the subtle and not so subtle changes I am seeing. Every thing is cutting
well except the more fatty tissues (breasts, colons, lipomas etc.) I have
listed my routine program below. I forgot to mention that we put our fatty
tissues in lilies fix (formaldehyde, alcohol and acetic acid)
For 3-5 hours prior to processing.
Station
Fluid
Time
1
Formalin 10%
60 mins
2
Formalin 10%
60 mins
3
70% Reagent alcohol
45 mins
4
95% Reagent alcohol
50 mins
5
95% Reagent alcohol
50 mins
6
100% Reagent alcohol
50 mins
7
100% Reagent alcohol
50 mins
8
100 % Reagent alcohol
50 mins
9
Xylene
45 mins
10
Xylene
45 mins
11
Empty/ paraffin bath
0
12
Paraffin
30 mins
13
Paraffin
30 mins
14
Paraffin
45 mins
15
Clean xylene
16
Clean alcohol
Cheryl Miller HT (ASCP)
Histology Supervisor
Physicians Laboratory,P.C.
Omaha, Ne.
402 738 5052
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