Hi Everyone,
I am working up some FISH staining protocols for cytology (cell culture) slides (eventually to be OCT and FFPE tissues, but I need to walk before I can run).
Most of the journals that I have read recommend using a fixative containing methanol and acetic acid (3:1). The DAKO probe I am using calls for the use of 3.7% fomaldehyde. I am wondering why acetone (or acetone/ethanol) is not the routine fixative for such a procedure. Can someone let me know why to choose one fixative over another and what the merits of methanol/glacial acetic acid would be?
Thanks in advance,
Kim
Kim Merriam, MA, HT(ASCP)
Cambridge, MA
---------------------------------
Never miss a thing. Make Yahoo your homepage.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
|