If this crystals are formalin-pigment they have reducing capacitiy.
Therefore with silverimpregnation techniques like Masson this would render
black spots. Also the PAS stain would give positivity.

Gudrun Lang
 
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754
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Von: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Jim
Manavis
Gesendet: Donnerstag, 28. Februar 2008 03:41
An: Histonet
Betreff: [Histonet] hb crystals

Dear Sir or Madam:

 

I am a PhD student studying intracerebral haemorrhage in the rat and have
found numerous crystals contained within the haemorrhage, which I presume
are haemoglobin crystals, although it would be nice to demonstrate this.
Pearl's stain doesn't work, presumably because the iron is ferrous and bound
tightly, and Lillie's method with ferricyanide and HCL likewise doesn't
work, presumably because the iron is bound. Our standard DAB technique for
immunohistochemistry doesn't seem to react either. How might I best
determine whether or not the crystals are haemoglobin crystals?

 

Yours Sincerely, 

 

Tim Kleinig

 

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