Re: [Histonet] trichrome and immunohistochemistry

From:Rene J Buesa

It can be done but it also depends on the epitope locaiotn. If it is nuclear you cannot use the iron hematoxylin, and if it is cytoplasmic you have to go with a very light cytoplasmic staining step.
  The only thing I wonder is why would you want to do that? IHC "standard" is running the procedure itself up to the DAB and ending with a very light hematoxylin (any regressive for 30 seconds with just blueing).
  René J.

Bernard Martin  wrote:
  Has anyone ever counterstained IHC with trichrome, or
heard of it being done? Is this even possible? Thanks!

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