Re: [Histonet] re/prostate quesitons

From:Matt Bancroft

The issue that I have found is even when one uses + charged slides, you still need to make sure that all the water is gone from under the tissue section.  When I cut needle biopsies (kidney, prostate, or breast) after I get the section on the slide I take my forceps tare a little slit in the corner of my sections and use a kim wipe to absorb the water.  I have not had a problem with sections falling off since I started doing this.  I also use HISTOBOND slides from StatLab medical products. These slides are very sticky (sometimes to much).
  Matt Bancroft HT(ASCP)
  Laboratory Manager

Joyce Cline  wrote:
  1. We do not have the same problem with our breast core biopsies.
2. We may have washing on block A and B but not on C,D or F and
washing on E, H, etc.
3. The biopsies have no drying artifact on the edges.
4. We use a low temp wax, Blue Ribbon paraffin for processing and
embedding. Our processor is set at 57.
5. The tissue sections fall off during H&E staining.
6. We do not use sponges, we use screened cassettes.
7. The Stay on is used alone, not with the charged slides.

Our pathologist that is our prostate specialist thinks each one of us is
doing something different when we cut. We have all discussed this matter
and can find no 
differences when we cut.

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