Debbie,

I've been using Amonia water for a long time, but with caution, it
isn't necessary for all tissues and the strength of the solution along
with the length of time could affect the H&E staining.  A general
solution strength is between .25 to 1.25% and for no longer than five
minutes (depending on the tissue type) should be done.

All slides should be drained of any excess water (when using plus
slides or coated slides - scoring of the paraffin around the tissue
will help release any trapped water) before heating.  Excess water
under the section will result in tissue loss and staining issues.

Vikki

On 2/12/08, WWmn916@aol.com  wrote:
> hello everyone
>
> two little detail questions
> 1) could soaking prefaced paraffin blocks a little too long in ammonia water
> effect the h+e staining of that slide causing a muddy h+e?
> 2)could leaving slides in 65-70 degree oven (about 30-45 minutes or longer)
> cause heat artifact and muddy the h+e stain?
>
> thanks
> deb king
>
>
>
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