Re: [Histonet] little things in histology

From:Rene J Buesa

Amonia water has been described as affecting the H&E staining. Ammonia water on the faced off block is less helpful than a gentle soaking in arm water, or rubbing the surface with a gauze impregnated in mineral oil.
  Heating the slides don't UNLESS they are not completely drained (absolutely no water between the section and the slide) before heating them.
  René J. wrote:
  hello everyone

two little detail questions
1) could soaking prefaced paraffin blocks a little too long in ammonia water 
effect the h+e staining of that slide causing a muddy h+e?
2)could leaving slides in 65-70 degree oven (about 30-45 minutes or longer) 
cause heat artifact and muddy the h+e stain?

deb king

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