Re: [Histonet] Technovit 9100 problems

From:Rene J Buesa

  You are describing a problem that is common when small subjects (and in your case also with high water contents) have been dehydrated too quickly and probably "cleared" too long resulting in a brittle subject difficult to section and to be kept on the slides.
  Remedies after the fact are abundant and usually useless. I advise you to check your processing protocol and optimize it to your study subjects. I am sure that you have plenty available to make some tests and get to an adequate protocol. Once you do that, you will be fine and will not need advises like the one you are seeking now.
  René J.

Barbara Verstraeten  wrote:

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