One more thought, are your slides fully dry? I find difficulties in 
focussing sometimes with slides which are still wet. It's better to 
allow time to dry, safer for your scope as well.

>
>
>Bernard Martin  wrote:
>   My IHC looks great at 5x, 10x, and 20x, but when I go
>up to 40x, it is a bit blurry. The objective is
>clean, as is the slide itself. I deparaffinate in
>clean xylene. Anyone run into this problem, and if
>so, how did you resolve it? Thanks!
>

-- 

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