Make sure that when you finish and dehydrate to coverslip, that dehydration is complete before clearing the section with xylene.
  René J. 

Bernard Martin  wrote:
  My IHC looks great at 5x, 10x, and 20x, but when I go
up to 40x, it is a bit blurry. The objective is
clean, as is the slide itself. I deparaffinate in
clean xylene. Anyone run into this problem, and if
so, how did you resolve it? Thanks!


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