Re: [Histonet] IHC unclear at 40x

From:"Andrea Hooper"

99/100 that's from a blurry objective. 40X commonly are swiped 
through oil or worse yet, through wet mounting media (heavens 
forbid). So I suggest that you reclean your objective, even though 
you think it's clean.

At 11:45 AM -0800 2/8/08, Bernard Martin wrote:
>My IHC looks great at 5x, 10x, and 20x, but when I go
>up to 40x, it is a bit blurry.  The objective is
>clean, as is the slide itself.  I deparaffinate in
>clean xylene.  Anyone run into this problem, and if
>so, how did you resolve it?  Thanks!


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