Hi Sharron:

    You can use acetaldehyde instead of paraldehyde but you must use 
three times as much (one paraldehyde dissociates to 3 acetaldehydes in 
the stain). See "Staining properties of aldehyde fuchsin analogs" by 
Buehner et al. J. Histochem. Cytochem 27(3);782-787, 1979.
    If you can get your pathologist to write an Rx for parladehyde get 
in the little ampoules so you always have fresh. Using acetaldehyde is 
easier! And make sure your "basic fucsin" is really pararosanaline.

Geoff

Genest, Sharon SktnHR wrote:
> I am having difficulty obtaining paraldehyde for this method does anyone
> have suggestions for and alternate method that does not use this? I
> already have Movat and Verhoeff but some Pathologists prefer the GAF.
>
> Does anyone have a supplier with a Canadian distributor that I can
> purchase control blocks for amoebae, spirochetes, micobacterium leprae? 
>
> Sharon Genest MLT
> Acting Technologist III
> Histology Saskatoon Health Region
> Phone: (306)655-8197
> Email: sharon.genest@saskatoonhealthregion.ca
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>   


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff@umdnj.edu
**********************************************



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